One Jerusalem, an organization headed by former minister Natan Sharansky and Yehiel Leiter, is expected to launch the campaign on Sunday ahead of the peace conference in Annapolis, Maryland.
The organization launched a similar campaign seven years ago during Ehud Barak's tenure as prime minister. Ehud Olmert, then mayor of Jerusalem, was the driving force behind that initiative.
Leading Israeli public figures, a number of US congressmen and hundreds of One Jerusalem activists are expected to attend a ceremony that will kick off the campaign at the Armon Hanatziv promenade in the capital.
Meanwhile, another campaign against the division of Jerusalem has been launched in the press and on the internet. The campaign's organizers plan to hand out 500,000 golden ribbons to residents and ask that they tie them to their cars as a show of support.