The woman sustained stab wounds to her upper body in the attack on Route 443, close to Maccabim Junction.
The suspected attacker, a 14-year-old Palestinian boy, was arrested by Border Police officers who were driving by at the time of the incident.
The teenager was apprehended while trying to escape.
Magen David Adom parademics and other emergency personnel treated the woman at the scene of the incident.
United Hatzalah volunteer EMT Yosef Horowitz, who was one of the first responders at the scene said: "Together with other other EMS (emergency medical services) personnel, I treated a young woman who suffered a stab wound to her upper body.
"She was in light to moderate condition. We also treated another person who sustained light injuries in the incident."