The number of dead includes 180 civilians and the number of wounded has risen to nearly 2,000, Moawiya Hassanei told AFP.
Also Wednesday night, Israel Air Force jets attacked government buildings across the Gaza Strip, including the parliament building. Hamas sources said the Education and Transportation ministries were completely destroyed in the strikes.
The Israel Defense Forces confirmed that the IAF had attacked several targets in the Strip overnight.
A raid on the house of the Rafah leader of Hamas' armed wing, the Izz al-Din al- Qassam Brigades, killed three neighbors, including one woman, he said.
Abu Anas Chabana was not at home at the time. The house was destroyed and nearby homes damaged.
An airstrike for the first time targeted the Justice Ministry in Gaza City, wounding, a medical source said.
A building belonging to a Hamas-linked group was also destroyed in the Maghazi refugee camp in the center of the Gaza Strip.
Two homes belonging to Hamas militants were destroyed in the central Nusseirat refugee camp, witnesses said. It was not immediately clear if anyone had been killed or injured.
A metalworking shop in Khan Younis was destroyed in a raid that wounded three, a military source said.
Another metalworking factory was targeted in Gaza City. Artillery fire from Israeli naval vessels also hit northern Gaza city, the medic said.
Reuters contributed to this report