Israeli jets struck a Hamas weapons factory in the northern Gaza Strip and two smuggling tunnels in the southern Strip in the early hours of Tuesday morning.
The strike came in response to the rocket that was fired at Israel from Gaza Monday evening.
Around 6:50 pm Monday, Palestinian gunmen from the Gaza Strip launched a Qassam rocket at the western Negev.
The rocket exploded in an open area between two kibbutzim. There were no reports of damage or injury.
The IDF Spokesperson's Unit said Tuesday's strike was in response to Monday's rocket.
"The IDF will not tolerate attacks from terror organizations on the State of Israel's territory and will continue to operate with a firm hand against any attempt to disrupt the calm in Israel's towns and the south of the country," an IDF statement said.
Meanwhile, two members of Hamas' military wing were killed in an explosion in a Gaza City neighborhood over night, medical sources and eyewitnesses reported.
Four others were injured in the incident. According to witnesses, the operatives were working to build a bomb when it exploded.
The IDF said it has no knowledge of the incident.