The ministers' security service, which consists of dozens of officers, has declared a wage dispute with Mikud, one of the government's personal security outsourced contractors.
The officers, who are represented by the General Federation of Workers in Israel (GFWI), claim that their work conditions and Mikud's wages are "disgraceful."
The Knesset Labor, Welfare and Health Committee is scheduled to debate the dispute later on Monday.
A position paper presented to the committee said that the company won the Knesset's tender despite previous complaints against its procedures, which the guards claim include 40-70 hour shifts.
The GFWI informed the Knesset that unless the matter is resolved it will declare a work dispute.
Committee Chairman MK Haim Katz (Likud )said that the committee will hear from both parties and will try to "bring this issue to a satisfactory end for both sides."
The Knesset spends some NIS 100 million (about $26.3) a year on ministers' security.
Mikud was unavailable for comment.